Assignment of SNP allelic configuration in polyploids using competitive allele-specific PCR: application to citrus triploid progeny

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Assignment of SNP allelic configuration in polyploids using competitive allele-specific PCR: application to citrus triploid progeny.

BACKGROUND Polyploidy is a major component of eukaryote evolution. Estimation of allele copy numbers for molecular markers has long been considered a challenge for polyploid species, while this process is essential for most genetic research. With the increasing availability and whole-genome coverage of single nucleotide polymorphism (SNP) markers, it is essential to implement a versatile SNP ge...

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Citrus (Rutaceae) SNP markers based on Competitive Allele-Specific PCR; transferability across the Aurantioideae subfamily1

UNLABELLED PREMISE OF THE STUDY Single nucleotide polymorphism (SNP) markers based on Competitive Allele-Specific PCR (KASPar) were developed from sequences of three Citrus species. Their transferability was tested in 63 Citrus genotypes and 19 relative genera of the subfamily Aurantioideae to estimate the potential of SNP markers, selected from a limited intrageneric discovery panel, for on...

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Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR ASP and Dideoxy Single Allele-Specific PCR DSASP methods. The DSASP is the proposed new method for allelic discrimination. ...

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Application of Allele Specific Markers in Determining Alleles at VRN-1 Loci in Bread Wheat Genotypes

    Wheat is the most important crop and is cultivated all over the world. Wheat has a wide range of adaptability to different climates. Vernalization genes are one of the important factors determining wheat adaptability. in wheat breeding program understanding the alleles at vernalization requirement gens are useful to introduce new cultivar for different climates. At the molecular level, the ...

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ژورنال

عنوان ژورنال: Annals of Botany

سال: 2013

ISSN: 1095-8290,0305-7364

DOI: 10.1093/aob/mct032